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2.6 In vitro cellular model: OGD/R in SH-SY5Y cells Human neuroblastoma SH-SY5Y cells were cultured in high-glucose Dulbeccos Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific, Waltham, MA, United States) and maintained in a 5% CO 2 incubator at 37C

This contrasts with testosterone replacement therapy, which does impair fertility and typically must be stopped when trying to conceive